The Chincoteague National Wildlife Refuge and Assateague Island National Seashore, VA are open the following hours throughout the year.

May through September - 5:00 a.m. - 10:00 p.m., Daily
October - 6:00 a.m. - 8:00 p.m., Daily
November through Mid-March - 6:00 a.m. - 6:00 p.m., Daily
Mid-March through April - 6:00 a.m. - 8:00 p.m., Daily

The Wildlife Loop is open during normal operating hours for walking and biking but is also open to drive daily from 3pm to dusk.

Entrance to the refuge is free if you are walking and biking or there are daily, weekly, and annual fees if you plan to drive your vehicle over. Annual military and senior passes are also available.